Coding

Part:BBa_K1882005:Design

Designed by: Milena Krach   Group: iGEM16_Tuebingen   (2016-10-14)


Phosphofructokinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon usage of this part was optimized to balance DNA synthesis and expression in l.Johnsonii. Therefore longterm and shortterm repeats had to be modified and biobrick restriction sites were removed. The part was modified for RFC10 at the prefix and suffix of the part.


Source

Lactobacillus johnsonii ATCC33200, NCBI reference number: ZP_04007664.1

References